Mouse Cerberus ELISA Kit

Antibodies.com supplies a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Cer1 in serum, plasma or other biological fluids. The assay uses a 96-well microplate pre-coated with an antibody specific for the target, with detection carried out using a biotinylated anti-Cer1 antibody, an HRP-Avidin conjugate, and a TMB substrate. The enzymatic reaction produces a blue product that turns yellow upon addition of the stop solution, and the absorbance is read at 450 nm on a microplate reader. Target concentration is calculated by reference to the standard curve. Sample types covered include serum, EDTA plasma, heparin plasma, cell culture supernatant, tissue homogenates, and cell lysates.

The kit reports a sensitivity of 11.7 pg/ml and a quantitative range of 31.2-2000 pg/ml, with a total assay time of 4 hours 30 minutes. Reported recovery averages from the manufacturer cover serum (80%-102%, mean 91%), EDTA plasma (81%-100%, mean 90%) and heparin plasma (80%-89%, mean 84%), alongside linearity data at 1:2, 1:4, 1:8 and 1:16 dilutions across the same sample matrices. Components are stored at +4°C or -20°C as indicated and must not be used past the stated expiration date. The product is intended for research use only and is not for diagnostic or therapeutic applications.