Mouse CCAAT/Enhancer Binding Protein Beta ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com designed for the in vitro quantitative determination of mouse CEBP beta in tissue homogenates, cell lysates and other biological fluids. The assay uses a pre-coated microplate, a biotinylated anti-CEBP beta detection antibody, an HRP-avidin conjugate and TMB substrate; absorbance is read at 450 nm on a colorimetric microplate reader, with the CEBP beta concentration calculated by reference to a standard curve. The stated assay range is 78.1-5000 pg/ml and the sensitivity is 33 pg/ml, with a total assay time of 4 hours 30 minutes. Spike-and-recovery values reported in the product information average 91% for serum (range 80-102%), 90% for EDTA plasma (range 81-100%) and 84% for heparin plasma (range 80-89%), and linearity has been characterised across 1:2 to 1:16 dilutions of serum, EDTA plasma and heparin plasma. Standards are prepared by serial dilution to 5000, 2500, 1250, 625, 312.5, 156.3 and 78.1 pg/ml. Kit components include a pre-coated microplate, lyophilised standard, detection solutions A and B, wash buffer concentrate, sample dilution buffer, TMB substrate, stop solution and plate sealers, stored at +4°C or -20°C as instructed. The kit is intended for research use only.