Mouse Calpain 10 ELISA Kit

A sandwich enzyme-linked immunosorbent assay (sELISA) from Antibodies.com, this Mouse Calpain 10 ELISA Kit is intended for the in vitro quantitative determination of mouse Calpain 10 in tissue homogenates or other biological fluids. The assay uses a 96-well microplate pre-coated with an antibody specific for Calpain 10, with detection achieved using a biotinylated anti-Calpain 10 antibody, HRP-avidin conjugate, and TMB substrate; absorbance is read at 450 nm in a microplate reader and concentration is calculated against a standard curve.

Reported sensitivity is <0.103 ng/ml with an assay range of 0.312-20 ng/ml and a total assay time of 4h 30m. Sample recovery (n=5) is documented for serum (83-100%, average 91%), EDTA plasma (90-103%, average 96%), and heparin plasma (89-102%, average 95%), with dilution linearity provided for 1:2, 1:4, 1:8, and 1:16 dilutions across the same sample types.

Kit components include a pre-coated 96-well microplate, lyophilised standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers. Components are stored at temperatures between +4°C and -20°C, and the kit should not be used past its expiration date.