Mouse Cadherin, Epithelial ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of mouse E Cadherin (epithelial cadherin) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

The assay employs the sandwich enzyme immunoassay technique using a pre-coated microtitre plate with an antibody specific for E Cadherin. Standards and samples are added to the wells, and captured E Cadherin is detected using a Biotinylated Anti-E Cadherin Antibody followed by HRP-Avidin conjugate. The reaction is visualised with TMB substrate, producing a blue product that changes to yellow upon addition of acidic stop solution, read at 450 nm in a colorimetric microplate reader. The assay has a measuring range of 0.312–20 ng/ml and a sensitivity of <0.078 ng/ml, with a total run time of approximately 4 hours 30 minutes.

Reported recovery values are 91% from serum (range 80–102%, n=5), 90% from EDTA plasma (range 81–100%, n=5), and 84% from heparin plasma (range 80–89%, n=5). Dilution linearity has been demonstrated at 1:2, 1:4, 1:8, and 1:16 dilutions across serum, EDTA plasma, and heparin plasma samples. Kit components should be stored at +4°C or –20°C as instructed and the kit should not be used past its expiration date.