Mouse Brain Finger Protein ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the quantitative determination of mouse Brain Finger Protein in tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) with colorimetric detection read at 450 nm in a microplate reader.

The reported assay range is 0.156–10 ng/ml with a sensitivity of <0.066 ng/ml, and the total assay time is 4 hours 30 minutes. Standards and samples are captured by an immobilized antibody, then detected using a biotinylated anti-target antibody and HRP-avidin conjugate. TMB substrate produces a blue product that turns yellow upon addition of stop solution, with the yellow intensity proportional to analyte concentration and quantifiable against the standard curve.

Kit components are stored at +4°C or -20°C as instructed and should not be used past the expiration date.