Mouse Birc5 ELISA Kit

From Antibodies.com, this sandwich ELISA kit enables quantitative in vitro determination of mouse Survivin (Birc5) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The format is a pre-coated microplate arranged as 12 x 8 well strips, using a biotinylated anti-Survivin detection antibody followed by HRP-Avidin and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

Manufacturer-reported performance includes a standard curve range of 15.6–1000 pg/ml and a sensitivity of less than 5.6 pg/ml, with a total assay time of 4 h 30 min. Spike-and-recovery averaged 95% in serum (n=5, range 90–101%), 95% in EDTA plasma (n=5, range 91–99%) and 94% in heparin plasma (n=5, range 90–99%), with dilution linearity reported across 1:2 to 1:16 for each matrix. Supplied components include lyophilized standard, detection solutions A and B, 30× wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, stored at +4 °C or −20 °C as instructed.