Mouse B-Cell Activating Factor ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of mouse B-Cell Activating Factor (BAFF) in serum, plasma, tissue homogenates and other biological fluids. The assay employs a pre-coated 96-well microplate (12 x 8 well strips) and detection is read by absorbance at 450 nm on a colorimetric microplate reader. Total assay time is approximately 4 hours 30 minutes.

The stated assay range is 1.56–100 pg/ml with a sensitivity of 0.55 pg/ml. Kit components are stored at +4 °C or -20 °C as instructed and must not be used past the expiration date. Reported recovery values include serum (range 80%–102%, average 91%), EDTA plasma (range 81%–100%, average 90%) and heparin plasma (range 80%–89%, average 84%), with dilution linearity reported across 1:2 to 1:16 dilutions for each sample type.

Components supplied include a pre-coated 96-well microplate, lyophilized standard, Detection Solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and adhesive plate sealers.