Mouse ATPase, H+/K+ Exchanging Beta Polypeptide ELISA Kit

A sandwich ELISA designed for the quantitative in vitro determination of mouse Hydrogen Potassium ATPase beta (ATP4B) in tissue homogenates or other biological fluids, supplied by Antibodies.com within the Assays and Kits category. The assay uses a pre-coated 96-well microplate (12 x 8 well strips), a biotinylated detection antibody, HRP-avidin conjugate, and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader. Total assay time is approximately 4 hours 30 minutes.

Reported performance characteristics include a sensitivity of <0.066 ng/ml and an assay range of 0.156–10 ng/ml. Spike-and-recovery testing (n=5) gave average recoveries of 91% in serum, 94% in EDTA plasma, and 94% in heparin plasma. Across dilutions from 1:2 to 1:16, recovery ranges were 80–110% in serum, 70–108% in EDTA plasma, and 79–110% in heparin plasma. Kit components include a pre-coated microplate, lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers. Components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.