Mouse ATP Binding Cassette Transporter A1 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com is designed for the in vitro quantitative determination of mouse ATP Binding Cassette Transporter A1 (ABCA1) in serum, plasma, tissue homogenates or other biological fluids. The assay employs a pre-coated 96-well microplate arranged as 12 x 8 well strips, with an antibody specific for ABCA1 immobilised on the plate surface. Standards and test samples are added to the wells and the ABCA1 present is captured by the immobilised antibody; after washing, a Biotinylated Anti-ABCA1 Antibody is added, followed by an HRP-Avidin conjugate and a TMB substrate step terminated with an acidic stop solution. The absorbance is read at 450 nm in a colorimetric microplate reader and the ABCA1 concentration is calculated against the standard curve.

The manufacturer reports a product range of 0.156–10 ng/ml and a sensitivity of 0.064 ng/ml, with a total assay time of 4 hours 30 minutes. The kit is stated to be compatible with serum, EDTA plasma, heparin plasma and tissue homogenates, and components are stored at +4°C or -20°C as instructed; the kit should not be used past its expiration date.

Reported spike-and-recovery data (n=5) cover serum at 80–102% with a 91% average, EDTA plasma at 81–100% with a 90% average, and heparin plasma at 80–89% with an 84% average. Dilution-linearity data across 1:2, 1:4, 1:8 and 1:16 dilutions are also provided in the manufacturer source.