Mouse Apolipoprotein B ELISA Kit

From Antibodies.com, this Mouse Apolipoprotein B ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Apolipoprotein B in serum, plasma or other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) and follows a standard sandwich immunoassay workflow, employing a biotinylated anti-Apolipoprotein B detection antibody and an HRP-avidin conjugate. After incubation with TMB substrate, the colour intensity is read at 450 nm on a colorimetric microplate reader.

The assay has a measuring range of 3.12–200 ng/ml with a sensitivity of <1.22 ng/ml, and a total assay time of 4 hours 30 minutes. Kit components should be stored at +4°C or -20°C as instructed and the kit should not be used past its expiration date. Spike-and-recovery performance has been validated in mouse serum (range 80–102%, average 91%), EDTA plasma (81–100%, average 90%) and heparin plasma (80–89%, average 84%), with linearity demonstrated across dilutions from 1:2 to 1:16 for each sample type.