Mouse Apolipoprotein A2 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is intended for the in vitro quantitative determination of mouse Apolipoprotein A II/ApoA-II in serum, plasma or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, onto which an antibody specific for Apolipoprotein A II/ApoA-II has been immobilised. Standards and test samples are added to the wells, followed by a biotinylated anti-Apolipoprotein A II/ApoA-II detection antibody and an HRP-Avidin conjugate. A TMB substrate reaction produces a yellow colour upon addition of acidic stop solution, with absorbance read at 450 nm on a microplate reader and analyte concentration calculated against the standard curve.

Reported sensitivity is <28 ng/ml with an assay range of 78.1-5000 ng/ml, and the total assay time is 4h 30m. Detection is colourmetric on a standard microplate reader platform, and kit components are stored at +4°C or -20°C as instructed.

Spike-and-recovery averaged 91% in serum (range 80-102%, n=5), 90% in EDTA plasma (range 81-100%, n=5) and 84% in heparin plasma (range 80-89%, n=5). Linearity upon dilution from 1:2 to 1:16 was demonstrated across serum (74-107%), EDTA plasma (79-108%) and heparin plasma (82-105%) sample matrices.