Mouse Angiogenic Factor With G Patch And FHA Domains 1 ELISA Kit

This sandwich ELISA from Antibodies.com is designed for the in vitro quantitative determination of mouse AGGF1 (Angiogenic Factor With G Patch And FHA Domains 1) in serum, plasma, tissue homogenates, cell lysates or other biological fluids. The kit employs a pre-coated 96-well microplate with colorimetric detection read at 450 nm, using a biotinylated anti-AGGF1 antibody and an HRP-avidin conjugate with TMB substrate and acidic stop solution. The total assay time is approximately 4 hours 30 minutes.

Reactivity: Mouse. Sensitivity: 34 pg/ml. Assay range: 78.1-5000 pg/ml. Recovery has been validated in serum (n=5, range 80%-102%, average 91%), EDTA plasma (n=5, range 81%-100%, average 90%) and heparin plasma (n=5, range 80%-89%, average 84%). Linearity was demonstrated in serum, EDTA plasma and heparin plasma across serial dilutions from 1:2 to 1:16. Kit components are stored at +4°C or -20°C as instructed, and the product is intended for research use only.