Mouse Amine Oxidase, Copper Containing 3 ELISA Kit

From Antibodies.com, this Assays and Kits product is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) intended for the in vitro quantitative determination of mouse Amine Oxidase, Copper Containing 3 (VAP1) in serum, plasma, tissue homogenates or other biological fluids. The assay uses the sandwich enzyme immunoassay technique with a target-specific antibody pre-coated onto the microplate. Standards and samples are added to the wells, followed by a Biotinylated Anti-VAP1 Antibody and an HRP-Avidin conjugate. TMB substrate is then added, producing a blue-coloured product that changes to yellow upon addition of the stop solution. Absorbance is read at 450 nm in a colorimetric microplate reader, and target concentration is calculated by reference to the standard curve.

Sensitivity is 0.054 ng/ml with an assay range of 0.156–10 ng/ml, and the total assay time is 4 hours 30 minutes. Reported recovery values across n=5 samples are an average of 91% (range 80–102%) for serum, 90% (range 81–100%) for EDTA plasma, and 84% (range 80–89%) for heparin plasma. Linearity has been demonstrated at sample dilutions of 1:2, 1:4, 1:8, and 1:16. Kit components should be stored at +4°C or −20°C as instructed, and the product should not be used past its expiration date.