Mouse Alpha-Fetoprotein ELISA Kit

The Mouse Alpha-Fetoprotein ELISA Kit from Antibodies.com is a quantitative immunoassay for measuring AFP, a ~70 kDa glycoprotein normally secreted by the fetal liver and yolk sac. AFP expression is largely silenced after birth but is reactivated in adult mice during hepatocellular injury, regeneration, and in hepatocellular carcinoma or teratoma models, making it a useful serum biomarker in liver-disease and tumor-biology research.

This in vitro, research-use-only assay is supplied as a complete kit containing a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated AFP standard, and wash and substrate buffers. The protocol accommodates small-volume mouse samples, with reagents equilibrated to room temperature before the run and the unused microplate stored refrigerated between sessions to preserve antibody activity. The kit supports quantitative work in transgenic, knockout, and chemically induced liver-injury models.