Mouse Alpha-Fetoprotein ELISA Kit

This quantitative immunoassay kit from Antibodies.com enables precise measurement of alpha-fetoprotein (AFP) in mouse biological specimens. AFP is a 70 kDa glycoprotein expressed in the developing fetal liver and reactivated in adult models of hepatocellular carcinoma, hepatoblastoma, and germ cell tumors, making it a widely tracked biomarker in murine oncology, toxicology, and developmental biology studies.

The kit is supplied as a 48-well microplate strip format with pre-coated capture antibody, biotinylated detection antibody, streptavidin-HRP conjugate, and calibrated AFP standard. Each run requires a small sample volume introduced into coated wells, producing a standard curve suitable for interpolation against unknown specimens. Researchers in liver cancer xenograft models, regenerative studies, and prenatal screening protocols rely on AFP quantification to track disease progression, treatment response, and tissue-specific gene expression.

Reactivity is validated specifically for mouse alpha-fetoprotein, with the assay optimized to distinguish endogenous murine AFP from related serum proteins. The complete reagent set includes wash buffer, substrate solution, and stop solution for use in standard microplate readers. Refrigerated storage of all components between uses is recommended to maintain reagent activity, and each component is lot-traceable to ensure reproducible inter-assay performance across multiple experiments.