Mouse Alkaline Sphingomyelinase ELISA Kit

A sandwich ELISA kit from Antibodies.com is intended for the quantitative determination of mouse analytes in tissue homogenates and other biological fluids. The catalogue entry lists the target as Alkaline Sphingomyelinase, while the manufacturer's accompanying protocol refers to the protein target as NPP-7.

The assay uses a pre-coated 96-well microplate (12 x 8 well strips), a biotinylated detection antibody, an HRP-avidin conjugate, and a TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader. Total assay time is 4 hours 30 minutes. The manufacturer quotes a sensitivity of 0.051 ng/ml and an assay range of 0.156-10 ng/ml. Kit components are stored at +4°C or -20°C as instructed.

Supplied components include the pre-coated microplate strips, lyophilized standard (2 vials), Detection Solution A (120 μl), Detection Solution B (120 μl), 30X Wash Buffer (20 ml), Sample Dilution Buffer (45 ml), TMB Substrate (9 ml), Stop Solution (6 ml), and 5 adhesive plate sealers. Manufacturer-reported average recovery values are 96% for serum (90-102%, n=5), 96% for EDTA plasma (90-103%, n=5), and 94% for heparin plasma (91-98%, n=5). Reported dilution linearity across 1:2 to 1:16 dilutions spans 72-108% across the three sample types.