Mouse Aldehyde Dehydrogenase 1 Family, Member A1 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit from Antibodies.com is intended for the in vitro quantitative determination of mouse Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) in serum, plasma, tissue homogenates or other biological fluids. The assay is run on a pre-coated 96-well microplate arranged as 12 x 8 well strips and is read on a colorimetric microplate reader at 450 nm.

The manufacturer states an assay range of 0.156–10 ng/ml and a sensitivity of <0.059 ng/ml, with a total assay time of 4 h 30 m. Spiked-recovery data from the manufacturer show an average recovery of 91% in serum (n=5, range 80–102%), 91% in EDTA plasma (n=5, range 81–101%) and 84% in heparin plasma (n=5, range 80–89%). Dilution linearity is reported at 1:2, 1:4, 1:8 and 1:16 for serum, EDTA plasma and heparin plasma (n=5 each), with the corresponding percentage ranges listed in the source. Kit components are stored at +4 °C or −20 °C as instructed, and the product must not be used past its expiration date.