Mouse Alcohol Dehydrogenase 1 ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of mouse Alcohol Dehydrogenase 1 in serum, plasma, tissue homogenates or other biological fluids. The assay uses a pre-coated microplate, a biotinylated anti-target antibody, an HRP-avidin conjugate and TMB substrate, with absorbance measured at 450 nm on a colorimetric microplate reader. It is categorized under Assays and Kits and is reactive with mouse samples.

Quantitative performance figures include a standard curve range of 0.312–20 ng/ml and a sensitivity of <0.09 ng/ml. The platform is a pre-coated 96-well microplate arranged as 12 x 8 well strips, and the total assay time is 4 hours 30 minutes. Spiked recovery in serum (n=5) averaged 91% (range 80–102%), in EDTA plasma (n=5) averaged 90% (range 81–100%), and in heparin plasma (n=5) averaged 84% (range 80–89%). Dilution linearity was reported at 1:2, 1:4, 1:8 and 1:16 across the same matrices.

Components should be stored at +4°C or -20°C as instructed and the kit should not be used past its expiration date. The product has been cited in at least one publication in the alcohol-related liver disease research field.