Mouse Alanine Aminotransferase ELISA Kit

A sandwich enzyme-linked immunosorbent assay (sELISA) from Antibodies.com is designed for the in vitro quantitative determination of mouse Alanine Transaminase in serum, plasma, tissue homogenates, cell lysates or other biological fluids. The assay employs a pre-coated 96-well microplate configured as 12 x 8 well strips and is read on a colorimetric microplate reader at 450 nm, with a total assay time of 4h 30m.

The standard curve covers a product range of 0.156–10 ng/ml with a reported sensitivity of 0.057 ng/ml. Reagent components are stored at +4°C or −20°C as instructed and the kit should not be used past its expiration date. The product is suitable for serum, plasma, cell lysates and tissue homogenates, and has been cited in 1 publication.

Manufacturer-reported recovery values average 91% for serum (n=5, range 80–102%), 90% for EDTA plasma (n=5, range 81–100%) and 84% for heparin plasma (n=5, range 80–89%). Dilution linearity at 1:2, 1:4, 1:8 and 1:16 spans 87–91%, 87–107%, 74–101% and 92–97% for serum (n=5); 90–105%, 84–101%, 90–101% and 79–108% for EDTA plasma (n=5); and 84–95%, 92–105%, 82–105% and 89–91% for heparin plasma (n=5).