Mouse Adrenomedullin ELISA Kit

A sandwich ELISA from Antibodies.com designed for the in vitro quantitative determination of mouse Adrenomedullin/ADM in serum, plasma or other biological fluids. The assay uses a 96-well microplate pre-coated with an antibody specific for Adrenomedullin/ADM, with detection via a biotinylated anti-Adrenomedullin/ADM antibody and an HRP-avidin conjugate, visualised using a TMB substrate. The colour change is measured at 450 nm on a colorimetric microplate reader and the Adrenomedullin/ADM concentration is calculated from a standard curve.

Reactivity is reported as Mouse, with an assay range of 15.6-1000 pg/ml and a sensitivity of 5.31 pg/ml. Total assay time is approximately 4 h 30 min. Reported recovery values (n=5) are 80-102% for serum (average 91%), 81-100% for EDTA plasma (average 90%) and 80-89% for heparin plasma (average 84%). Dilution linearity data are provided for serum, EDTA plasma and heparin plasma across 1:2, 1:4, 1:8 and 1:16 dilutions. Kit components include a pre-coated 96 well microplate (12 x 8 well strips), lyophilised standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, with storage at +4°C or -20°C as instructed.