Mouse Adiponectin Receptor 1 ELISA Kit

A sandwich enzyme-linked immunosorbent assay from Antibodies.com enables the in vitro quantitative determination of mouse Adiponectin Receptor 1 (ADIPOR1) in serum, plasma, and other biological fluids. The kit uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with colourmetric detection read at 450 nm on a microplate reader. Total assay time is 4 hours 30 minutes.

According to manufacturer specifications, the assay has a stated range of 3.12–200 ng/ml and a sensitivity of <1.17 ng/ml. Reported recovery values (n=5) average 91% for serum (range 80%–102%), 91% for EDTA plasma (range 81%–101%), and 84% for heparin plasma (range 80%–89%). Dilution linearity data is provided across 1:2, 1:4, 1:8, and 1:16 dilutions for serum, EDTA plasma, and heparin plasma samples.

Kit components include a pre-coated 96-well microplate, lyophilised standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers. Components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.