Mouse Adenosine Kinase ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of mouse Adenosine Kinase (ADK) in tissue homogenates and other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with a biotinylated anti-ADK detection antibody, HRP-avidin conjugate and TMB substrate; the reaction is read at 450 nm on a colorimetric microplate reader.

Sensitivity is stated as <0.054 ng/ml and the working range as 0.156–10 ng/ml, with a total assay time of 4h 30m. Recovery has been demonstrated in serum (80–102%, average 91%), EDTA plasma (81–100%, average 90%) and heparin plasma (80–89%, average 84%), and linear dilution behaviour has been confirmed from 1:2 to 1:16 across these matrices. Kit components are stored at +4°C or -20°C as instructed.