Mouse Acetylcholinesterase ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is designed for the in vitro quantitative determination of mouse Acetylcholinesterase in serum, plasma, tissue homogenates or other biological fluids. The kit employs the sandwich enzyme immunoassay technique on a pre-coated microplate platform. An antibody specific for Acetylcholinesterase is immobilised on the plate; standards and samples are added, followed by a Biotinylated Anti-Acetylcholinesterase Antibody and an HRP-Avidin conjugate. TMB substrate produces a blue colour that turns yellow upon addition of acidic stop solution, with absorbance read at 450 nm in a microplate reader.

The reported sensitivity is 0.32 ng/ml and the assay range is 0.781–50 ng/ml. Average recovery values across sample types are: serum 93% (range 85–101%, n=5), EDTA plasma 92% (86–99%, n=5), and heparin plasma 94% (87–101%, n=5). Dilution linearity has been evaluated at 1:2, 1:4, 1:8 and 1:16. The detection antibody is a rabbit IgG raised against recombinant protein, supplied in liquid form in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Total assay time is approximately 4 h 30 min. Kit components should be stored at +4 °C or −20 °C as instructed; do not use past the expiration date. Distributed by ARP American Research Products, Inc., the product is sold for laboratory research use only and is not intended for human or animal therapeutic or diagnostic applications.