Mouse A Disintegrin And Metalloprotease 8 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com intended for the in vitro quantitative determination of mouse A Disintegrin And Metalloprotease 8 in serum, plasma, tissue homogenates or other biological fluids.

The assay employs a pre-coated microplate with immobilised capture antibody. Standards and test samples are added, followed by a Biotinylated Anti-target Antibody and HRP-Avidin conjugate. TMB substrate is then used to visualise the HRP enzymatic reaction, producing a blue-coloured product that changes to yellow upon addition of acidic stop solution. Absorbance is read at 450 nm. Reported sensitivity is <5.7 pg/ml, with an assay range of 15.6–1000 pg/ml and a total assay time of 4 hours 30 minutes.

Recovery values reported are: serum (n=5) 80–102%, average 91%; EDTA plasma (n=5) 81–100%, average 90%; heparin plasma (n=5) 80–89%, average 84%. Linearity ranges across 1:2 to 1:16 dilutions are provided for each sample type. Kit components include lyophilised standard (2 vials), Detection Solution A (120 μl), Detection Solution B (120 μl), 30X Wash Buffer (20 ml), Sample Dilution Buffer (45 ml), TMB Substrate (9 ml), Stop Solution (6 ml), and 5 adhesive plate sealers. Components are stored at –20°C or +4°C as indicated, and the kit should not be used past its expiration date.