Monkey Tissue Inhibitors Of Metalloproteinase 3 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) supplied by Antibodies.com for the in vitro quantitative determination of monkey Tissue Inhibitor of Metalloproteinase 3 (TIMP3). The kit is suitable for use with serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

The assay uses a pre-coated microplate (12 x 8 well strips), a biotinylated anti-TIMP3 detection antibody, an HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm in a colorimetric microplate reader. The stated product range is 0.312-20 ng/ml and sensitivity is 0.108 ng/ml; total assay time is 4h 30m. Components include a lyophilized standard, Detection Solution A, Detection Solution B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and plate sealers, to be stored at +4°C or -20°C as instructed.

Reported spike-and-recovery averages are 91% for serum (range 80%-102%), 90% for EDTA plasma (range 81%-100%) and 84% for heparin plasma (range 80%-89%), with linearity-of-dilution data supplied for 1:2, 1:4, 1:8 and 1:16 sample dilutions across the same matrices.