Monkey Plasminogen Activator, Urokinase Receptor ELISA Kit

The pre-coated strip plate format (12 removable eight-well strips) lets researchers run a partial plate when fewer than 48 samples are queued, cutting waste on small cohort days. Reconstitute each lyophilized standard using the volume specified on the supplied certificate of analysis (typically 200 µL per vial) to reach the top calibrator, then produce serial two-fold dilutions in assay buffer to generate a seven-point standard curve.

Validated sample types include cynomolgus and rhesus macaque serum, EDTA plasma, tissue homogenate, and cell culture supernatant from stimulated peripheral blood mononuclear cells, with dilution linearity generally within 15% when serum is pre-diluted 1:2 or further. Spike-and-recovery in rhesus plasma typically falls between 85 and 110%, supporting quantitative interpretation in disease-model endpoints. Reported intra-assay CV stays under 8% and inter-assay CV remains under 10% across the working range, and the matched antibody pair shows no significant cross-reactivity with the murine uPAR ortholog, supporting clean discrimination between host PLAUR signal and engrafted human tumor PLAUR in xenograft macaque studies.

For reliable long-term performance, aliquot the reconstituted standard and detection reagent into single-use vials before freezing, keep unused strips sealed with the desiccant pouch in place, and bring all components to room temperature before each run. Antibodies.com provides protocol troubleshooting and curve-fitting guidance with every shipment.