Methadone-HRP Antigen

Prepared as a ready-to-use liquid in a 1 ml vial, this HRP-labeled methadone conjugate from Antibodies.com functions as a coating reagent or competitive tracer in immunoassay development. The horseradish peroxidase label delivers enzymatic signal amplification, producing a chromogenic readout on standard microplate readers when paired with substrates such as TMB in ELISA plate coating, competitive inhibition, and antibody screening workflows. Clinical toxicology laboratories, workplace drug-screening programs, and forensic analysts use this conjugate to develop assays that detect methadone exposure in urine, oral fluid, or serum samples following validated extraction procedures.

Beyond standard ELISA plate coating, the reagent supports lateral flow assay prototyping, competitive immunoassay optimization, and antibody pair characterization during assay research. The liquid format removes the reconstitution step required by lyophilized conjugates and supports direct dilution into assay buffers. Refrigerated storage preserves enzymatic activity over the stated shelf life, with brief equilibration to room temperature recommended before dispensing. Each lot is qualified for use in methadone immunoassays, and the product is supplied for research and in vitro diagnostic development only.