Human Zinc Ribbon Domain Containing Protein 1 ELISA Kit

Manufactured by Antibodies.com, this Sandwich ELISA kit is designed for the in vitro quantitative determination of human Zinc Ribbon Domain Containing Protein 1 (ZNDR1) in tissue homogenates, cell lysates, and other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips and follows a sandwich enzyme immunoassay format with a biotinylated detection antibody and an HRP-avidin conjugate, with signal generated by TMB substrate and read at 450 nm on a colorimetric microplate reader.

The kit reports an assay range of 0.312–20 ng/ml and a sensitivity of <0.107 ng/ml, with a total assay time of 4 h 30 min. Spike-and-recovery testing in human serum, EDTA plasma, and heparin plasma shows average recoveries of 91%, 90%, and 84% respectively across n=5 samples per matrix, and dilution linearity at 1:2 to 1:16 yields recoveries broadly within 74–108% across the same sample types. Kit components are stored at +4 °C or -20 °C and should not be used past the stated expiration date.