Human WISP1 ELISA Kit

From Antibodies.com, this Human WISP1 ELISA Kit is a quantitative sandwich Enzyme-Linked Immunosorbent Assay (sELISA) intended for the in vitro determination of human WISP1 in serum, plasma, tissue homogenates, and other biological fluids. The 4-hour, colorimetric assay is read at 450 nm, with absorbance directly proportional to the concentration of WISP1 captured by an immobilised antibody and detected via a biotinylated detection antibody, HRP-Streptavidin conjugate, and TMB substrate.

The kit is specific for WISP1, with no reported cross-reactivity with other analogs or variants, and operates over a working range of 31.25–2000 pg/mL with a sensitivity of less than 18.75 pg/mL. Serum and plasma samples should be diluted at least 1:2 with Sample Dilution Buffer prior to testing; validated dilutions for healthy serum and healthy plasma are 1:20–1:50. The kit is shipped at +4°C, with components stored at +4°C or –20°C as instructed in the Protocol Manual. Freeze/thaw cycles and use past the expiration date should be avoided.