Human Von Willebrand Factor A Domain Containing Protein 3A ELISA Kit

This sandwich ELISA from Antibodies.com is designed for the in vitro quantitative determination of human Von Willebrand Factor A Domain Containing Protein 3A in plasma samples.

The assay employs a pre-coated 96-well microplate arranged as 12 x 8 well strips. Standards and samples are added to the wells, followed by a biotinylated anti-target antibody, an HRP-avidin conjugate, and TMB substrate. Absorbance is read at 450nm in a colorimetric microplate reader and the target concentration is calculated from a standard curve. The stated assay range is 0.468-30 ng/ml with a sensitivity of <0.15 ng/ml. Total assay time is 4h 30m, and components should be stored at +4°C or -20°C as instructed; the kit should not be used past its expiration date.

Validation data cover serum, EDTA plasma, and heparin plasma, including recovery and dilution linearity at 1:2, 1:4, 1:8, and 1:16 dilutions. Kit contents include the pre-coated plate, lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers, each with its own storage condition.