Human Vitamin D Binding Protein ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com is intended for the in vitro quantitative determination of human Vitamin D Binding Protein in serum, plasma or other biological fluids. The format uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, read on a colorimetric microplate reader at 450 nm.

Performance characteristics include a sensitivity of <0.074 ng/ml and a product range of 0.156-10 ng/ml, with a total assay time of 4 hours 30 minutes. Reported average recovery values are 91% (80-102%, n=5) for serum, 90% (81-100%, n=5) for EDTA plasma and 84% (80-89%, n=5) for heparin plasma.

Samples and standards are added to wells pre-coated with a capture antibody specific for Vitamin D Binding Protein; bound analyte is detected using a biotinylated anti-Vitamin D Binding Protein antibody followed by HRP-avidin conjugate and TMB substrate, yielding a blue colour that becomes yellow after stop solution, with absorbance proportional to analyte concentration. Components supplied include the pre-coated microplate, lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers; kit components are stored at +4°C or -20°C as instructed and the kit is not to be used past its expiration date.