Human Vitamin D Binding Protein ELISA Kit

Distributed by Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) is designed for the in vitro quantitative determination of human Vitamin D Binding Protein in cell culture supernatant, serum and plasma. Reactivity is confirmed for human samples, with no significant cross-reactivity reported, and the assay can recognise both native and recombinant human Vitamin D Binding Protein.

The quantitative sandwich format uses microplate wells pre-coated with a monoclonal antibody specific for human Vitamin D Binding Protein. Standards or samples are added together with a biotin-linked detection antibody, followed by streptavidin-HRP and substrate solution. The resulting colour development, read on a colorimetric microplate platform, is proportional to the amount of Vitamin D Binding Protein bound by the immobilised antibody.

Quoted performance includes a measuring range of 78.13 – 5000 pg/ml and a sensitivity of 40.15 pg/ml. Kit components should be stored at +4°C and not used past the indicated expiry date. The product is offered under the Assays and Kits category.