Human VGF Nerve Growth Factor Inducible ELISA Kit

This sandwich ELISA kit from Antibodies.com enables the in vitro quantitative determination of human VGF (VGF Nerve Growth Factor Inducible) in serum, plasma, or other biological fluids. The assay employs a 96-well pre-coated microplate and a colorimetric microplate reader, with absorbance read at 450 nm. The workflow follows a standard sandwich immunoassay: an antibody specific for VGF is immobilised on the plate to capture analyte, a biotinylated anti-VGF antibody binds the captured VGF, and an HRP-avidin conjugate with TMB substrate produces a blue colour that turns yellow upon addition of stop solution. The intensity of the yellow product is proportional to the VGF concentration, which is calculated against a standard curve.

The kit reports a product range of 78.1-5000 pg/ml and a sensitivity of <32 pg/ml, with a total assay time of 4h 30m. Average recovery is 91% for serum (range 80-102%, n=5), 90% for EDTA plasma (range 81-100%, n=5), and 84% for heparin plasma (range 80-89%, n=5). Dilution linearity has been validated at 1:2, 1:4, 1:8, and 1:16, with serum recoveries ranging from 74-107%, EDTA plasma from 79-108%, and heparin plasma from 82-105% across the dilutions tested (n=5 per type).

Kit components include a pre-coated 96-well microplate, lyophilised standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers. Components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.