Human Vascular Endothelial Growth Factor 165 ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of human Vascular Endothelial Growth Factor 165 (VEGF165) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. It employs a pre-coated 96-well microplate configured as 12 x 8 well strips and is read on a colorimetric microplate reader at 450 nm. Reactivity is human-specific.

The stated product range is 15.6-1000 pg/ml with a sensitivity of <5.7 pg/ml, and the full assay procedure takes 4h 30m. Kit components should be stored at +4°C or -20°C as instructed and the kit should not be used past its expiration date.

Reported spike-and-recovery values (n=5) average 91% in serum (range 83-100%), 95% in EDTA plasma (range 90-100%) and 96% in heparin plasma (range 91-101%). Dilution linearity across 1:2, 1:4, 1:8 and 1:16 dilutions (n=5 each) yielded ranges of 81-99%, 90-102%, 77-104% and 88-95% for serum; 94-104%, 80-108%, 88-93% and 86-108% for EDTA plasma; and 76-100%, 92-99%, 88-105% and 85-99% for heparin plasma.