Human Urocanase Domain Containing Protein 1 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com is designed for the in vitro quantitative determination of human Urocanase Domain Containing Protein 1 (UROC1) in tissue homogenates, cell lysates or other biological fluids. It is intended for use with a colorimetric microplate reader, with absorbance read at 450 nm, and employs a pre-coated microplate platform arranged as 12 x 8 well strips.

The assay has a stated product range of 0.312–20 ng/ml and a sensitivity of 0.118 ng/ml, with a total assay time of 4h 30m. Kit components are stored at +4°C or −20°C as instructed, and the kit should not be used past its expiration date.

Reported recovery values for spiked samples fall within 80%–102% for serum (n=5), 81%–100% for EDTA plasma (n=5), and 80%–89% for heparin plasma (n=5). Dilution linearity across 1:2, 1:4, 1:8 and 1:16 dilutions is reported as 87–91%, 87–107%, 74–101% and 92–97% for serum (n=5); 90–105%, 84–101%, 90–101% and 79–108% for EDTA plasma (n=5); and 84–95%, 92–105%, 82–105% and 89–91% for heparin plasma (n=5).