Human UDP Glucuronosyltransferase 1 Family, Polypeptide A1 ELISA Kit

From Antibodies.com, this sandwich ELISA kit enables quantitative measurement of human UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) in tissue homogenates, cell lysates, and other biological fluids. The format uses a pre-coated microplate, a biotinylated anti-UGT1A1 detection antibody, and HRP-avidin with TMB substrate; the enzymatic reaction is stopped with acidic solution and read at 450 nm on a colorimetric microplate reader.

Reported assay sensitivity is 0.51 ng/ml with a working range of 1.56-100 ng/ml. Total assay time is 4h 30m, and components are stored at +4°C or -20°C according to instructions.

Spike-and-recovery data are provided for serum (average 91%, range 80-102%, n=5), EDTA plasma (average 91%, range 81-101%, n=5), and heparin plasma (average 84%, range 80-89%, n=5). Linearity across 1:2 to 1:16 dilutions is also documented for each sample type.