Human Ubiquitin Cross Reactive Protein ELISA Kit

Antibodies.com lists this sandwich ELISA as targeting Ubiquitin Cross Reactive Protein, with the manufacturer's product information describing it as a Human ISG15 ELISA Kit designed for the quantitative determination of human ISG15 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The assay employs a pre-coated 96-well microplate, a biotinylated anti-ISG15 detection antibody and an HRP–avidin conjugate, with TMB substrate producing a colour change read at 450 nm in a microplate reader.

According to the manufacturer's data, the kit has a sensitivity of <12.5 pg/ml and a working range of 31.2-2000 pg/ml, with a total assay time of 4 hours 30 minutes. Spike-and-recovery testing in serum, EDTA plasma and heparin plasma gave average recoveries of 91%, 90% and 84% respectively across five samples each, and linearity across dilutions from 1:2 to 1:16 remained within the ranges reported for each matrix.

The kit is supplied with a pre-coated microplate of 12 x 8-well strips, lyophilized standard, detection solutions A and B, 30X wash buffer concentrate, sample dilution buffer, TMB substrate, stop solution and adhesive plate sealers, to be stored at +4°C or -20°C as indicated for each component.