Human Tumor Protein p53 Binding Protein 1 ELISA Kit

This sandwich ELISA kit from Antibodies.com enables the in vitro quantitative determination of human 53BP1 (Tumor Protein p53 Binding Protein 1) in tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips; captured analyte is detected colourimetrically at 450 nm using a Biotinylated Anti-53BP1 Antibody and HRP-Avidin conjugate together with TMB substrate. Total assay time is 4 hours 30 minutes. Sensitivity is less than 0.138 ng/ml and the assay range is 0.312-20 ng/ml. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.

Reported spike-and-recovery values are: human serum (n=5) range 80%-102%, average 91%; EDTA plasma (n=5) range 81%-101%, average 91%; heparin plasma (n=5) range 80%-89%, average 84%. Dilution linearity across 1:2, 1:4, 1:8 and 1:16 dilutions is given as 87-91%, 87-107%, 74-101% and 92-97% for serum; 90-105%, 84-101%, 90-101% and 79-108% for EDTA plasma; and 84-95%, 92-105%, 82-105% and 89-91% for heparin plasma.