Human Tumor Necrosis Factor Ligand Superfamily, Member 4 ELISA Kit

A sandwich ELISA from Antibodies.com designed for the quantitative determination of human OX40L/TNFSF4 (Tumor Necrosis Factor Ligand Superfamily, Member 4) in serum, plasma, tissue homogenates, or other biological fluids. The assay uses a pre-coated 96-well microplate in a 12 x 8 well strip format, with a biotinylated anti-OX40L/TNFSF4 detection antibody, HRP-avidin conjugate, and TMB substrate. The plate is read by measuring absorbance at 450 nm in a colorimetric microplate reader, with a total run time of 4 hours 30 minutes.

The assay has a range of 15.6-1000 pg/ml and a sensitivity of 5.3 pg/ml (described in the source as <5.3 pg/ml). Recovery was evaluated in five samples per matrix: serum ranged from 80% to 102% with an average of 91%, EDTA plasma ranged from 81% to 101% with an average of 91%, and heparin plasma ranged from 80% to 89% with an average of 84%. Dilution linearity was assessed at 1:2, 1:4, 1:8, and 1:16 dilutions across serum, EDTA plasma, and heparin plasma (n=5 for each matrix).

Kit components include a pre-coated 96-well microplate, lyophilized standard, detection solutions A and B, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution, and plate sealers. Kit components are stored at +4°C or -20°C.