Human Tumor Necrosis Factor Ligand Superfamily, Member 13 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human APRIL/TNFSF13 (Tumor Necrosis Factor Ligand Superfamily, Member 13) in serum, plasma, tissue homogenates, or other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) and employs the sandwich enzyme immunoassay technique with a biotinylated detection antibody and HRP-avidin conjugate, with signal developed using TMB substrate and read on a colorimetric microplate reader.

Quantitative measurement is obtained by reading O.D. absorbance at 450 nm against a standard curve. The manufacturer reports a product range of 46.8–3000 pg/ml and a sensitivity of <16.64 pg/ml, with a total assay time of 4 h 30 min. Kit components are stored at +4°C or −20°C as instructed. Reactivity is human.

Validation data supplied by the manufacturer report the following recoveries (n = 5): serum average 91% (range 83–100%), EDTA plasma average 94% (range 90–98%), and heparin plasma average 95% (range 89–102%). Dilution linearity across 1:2 to 1:16 dilutions (n = 5) is reported as ranges of 79–110% for serum, 73–109% for EDTA plasma, and 77–110% for heparin plasma.