Human Trefoil Factor 1 ELISA Kit

A sandwich ELISA from Antibodies.com, listed with reactivity for human, is designed for the in vitro quantitative determination of the target protein in serum, plasma, tissue homogenates or other biological fluids. The Antibodies.com catalogue lists the target as Trefoil Factor 1, while the manufacturer's documentation identifies the protein target as human Estrogen Inducible Protein pS2. The assay uses the sandwich enzyme immunoassay technique on a pre-coated 96-well microplate arranged as 12 x 8 well strips, with a total assay time of 4h 30m. Following sample binding, a biotinylated detection antibody and an HRP-avidin conjugate are used, with detection achieved colorimetrically at 450nm using a TMB substrate. The kit reports a sensitivity of <54 pg/ml within a product range of 125-8000 pg/ml. Reported average recovery values across n=5 samples are 91% for serum (range 80-102%), 90% for EDTA plasma (range 81-100%), and 84% for heparin plasma (range 80-89%). Kit components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.