Human Transforming Growth Factor Beta Stimulated Protein Clone 22 ELISA Kit

From Antibodies.com, this sandwich ELISA kit enables in vitro quantitative determination of human TSC22 (Transforming Growth Factor Beta Stimulated Protein Clone 22) in tissue homogenates and other biological fluids. The assay uses a pre-coated microplate with antibody specific for TSC22, followed by incubation with a Biotinylated Anti-TSC22 Antibody and HRP-Avidin conjugate. Detection is colorimetric, with TMB substrate producing a blue product that turns yellow upon addition of stop solution; absorbance is read at 450 nm and concentration is calculated against a standard curve.

Reported performance figures from the manufacturer include a sensitivity of <0.113 ng/ml and an assay range of 0.312-20 ng/ml. The total assay time is 4 hours 30 minutes. Recovery data are: Serum (n=5) range 83-100%, average 91%; EDTA Plasma (n=5) range 90-103%, average 96%; Heparin Plasma (n=5) range 90-99%, average 94%. Dilution linearity at 1:2, 1:4, 1:8, and 1:16 is reported across the same three matrices.

Kit components include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers. Storage instructions indicate keeping components at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.