Human Topoisomerase I ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Topoisomerase I in tissue homogenates, cell lysates, or other biological fluids. It uses pre-coated microplate wells and a biotinylated detection antibody followed by an HRP-avidin conjugate, with a TMB substrate producing a yellow colour whose absorbance is read at 450 nm in a colorimetric microplate reader. The reported assay range is 1.56–100 ng/ml with a sensitivity of less than 0.60 ng/ml, and the typical run time is 4 hours 30 minutes. The kit is reactive with human samples and is listed under the Assays and Kits category.

Recovery has been evaluated in serum (range 80–102%, average 91%), EDTA plasma (range 81–100%, average 90%), and heparin plasma (range 80–89%, average 84%). Linearity has been tested at sample dilutions of 1:2, 1:4, 1:8, and 1:16, with serum recoveries of 87–91%, 87–107%, 74–101%, and 92–97%; EDTA plasma recoveries of 90–105%, 84–101%, 90–101%, and 79–108%; and heparin plasma recoveries of 84–95%, 92–105%, 82–105%, and 89–91%. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.