Human Tolloid Like Protein 1 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human TLL1 (Tolloid Like Protein 1) in serum, plasma, tissue homogenates and other biological fluids. The assay employs a pre-coated microplate, a biotinylated anti-TLL1 detection antibody, an HRP-avidin conjugate and TMB substrate for colorimetric readout.

Quantitation is achieved by reading absorbance at 450 nm on a colorimetric microplate reader and referring to the standard curve. The assay reports a measuring range of 0.625-40 ng/ml and a sensitivity of <0.216 ng/ml, with a total assay time of 4h 30m. Provided components include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution and plate sealers, with storage temperatures specified per component.

Validation data are reported for serum (n=5, average recovery 91%), EDTA plasma (n=5, average recovery 90%) and heparin plasma (n=5, average recovery 84%), with linearity documented across dilutions from 1:2 to 1:16 for each sample type. The kit is intended for research use only.