Human Toll Like Receptor 4 ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay enables in vitro quantitative measurement of human Toll Like Receptor 4 (TLR4) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit uses a 96-well microtiter plate pre-coated with a TLR4-specific capture antibody. Standards and samples are added, followed by a biotinylated anti-TLR4 detection antibody and HRP-Avidin, with TMB substrate yielding a yellow product read at 450 nm. The reported assay range is 0.156–10 ng/ml and sensitivity is 0.057 ng/ml, with a total assay time of 4 hours 30 minutes. Kit components are stored at +4°C or -20°C as instructed. Reported recovery in undiluted samples spans serum 80–102% (average 91%), EDTA plasma 81–100% (average 90%), and heparin plasma 80–89% (average 84%).