Human Tissue Inhibitors Of Metalloproteinase 4 ELISA Kit

This sandwich ELISA kit from Antibodies.com is intended for the in vitro quantitative determination of human TIMP4 in serum, plasma, tissue homogenates, cell lysates, or other biological fluids. The assay employs a pre-coated 96-well microplate (12 x 8 well strips) together with a biotinylated anti-TIMP4 detection antibody and an HRP-avidin conjugate, with signal developed using TMB substrate and read by measuring the optical density of the resulting yellow product.

Quantitative results are obtained by reading absorbance at 450 nm on a colorimetric microplate reader against a standard curve. The manufacturer reports a sensitivity of <0.058 ng/ml and an assay range of 0.156–10 ng/ml, with a total assay time of 4 hours 30 minutes. Reported sample recovery values are: serum 80%–102% (average 91%, n=5), EDTA plasma 81%–100% (average 90%, n=5), and heparin plasma 80%–89% (average 84%, n=5). Kit components are stored at +4°C or -20°C as instructed, and the kit is for research use only.