Human Thyroid Transcription Factor 1 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com enables the in vitro quantitative determination of human Thyroid Transcription Factor 1 (TTF1) in tissue homogenates, cell lysates or other biological fluids. The kit uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, a Biotinylated Anti-TTF1 Antibody, an HRP-Avidin conjugate and a TMB substrate; absorbance is read at 450 nm on a colorimetric microplate reader. Total assay time is 4h 30m, with a reported product range of 0.156–10 ng/ml and a sensitivity of <0.062 ng/ml. Kit components are stored at +4°C or -20°C as instructed. Spike-and-recovery performance was reported as: serum (n=5) 80–102%, average 91%; EDTA plasma (n=5) 81–100%, average 90%; heparin plasma (n=5) 80–89%, average 84%.