Human Thymus Expressed Chemokine ELISA Kit

From Antibodies.com, this sandwich enzyme-linked immunosorbent assay (sELISA) is designed for the in vitro quantitative determination of human CCL25 (Thymus Expressed Chemokine) in serum, plasma or other biological fluids. The assay uses a 96-well pre-coated microplate (12 x 8 well strips), a biotinylated anti-CCL25 detection antibody, HRP-avidin conjugate, and TMB substrate, with absorbance read at 450nm on a colorimetric microplate reader.

Stated specifications include a sensitivity of <11.5 pg/ml, an assay range of 31.2-2000 pg/ml, and a total assay time of 4h 30m. Reported average recovery (n=5) is 91% for serum (range 80%-102%), 90% for EDTA plasma (range 81%-100%), and 84% for heparin plasma (range 80%-89%). Dilution linearity is provided across 1:2, 1:4, 1:8, and 1:16 dilutions for each sample matrix. The kit includes a pre-coated 96 well microplate, lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers, to be stored at +4°C or -20°C as instructed.