Human Thrombospondin 1 ELISA Kit

From Antibodies.com, this sandwich enzyme-linked immunosorbent assay kit is designed for the in vitro quantitative determination of human Thrombospondin 1 in plasma samples. The format uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with colorimetric readout at 450 nm on a microplate reader. An antibody specific for Thrombospondin 1 is immobilized on the plate, and bound target is detected using a biotinylated anti-Thrombospondin 1 antibody, HRP-avidin conjugate, and TMB substrate.

The assay has a reported sensitivity of <0.11 ng/ml and an assay range of 0.25-16 ng/ml, with a total run time of 4 hours 30 minutes. Reported spike-and-recovery values (n=5) average 91% in serum (range 80-102%), 90% in EDTA plasma (range 81-100%), and 84% in heparin plasma (range 80-89%). Dilution linearity at 1:2, 1:4, 1:8, and 1:16 dilutions falls between 74% and 108% across serum, EDTA plasma, and heparin plasma samples. Kit components include a lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers, stored at +4°C or -20°C as indicated.