Human Thioredoxin Binding Protein 2 ELISA Kit

From Antibodies.com, this is a sandwich enzyme-linked immunosorbent assay (ELISA) for the in vitro quantitative determination of human TXNIP (Thioredoxin Binding Protein 2) in tissue homogenates, cell lysates, or other biological fluids.

The assay uses a pre-coated microplate read at 450nm on a colourmetric microplate reader. Detection chemistry employs a biotinylated anti-TXNIP antibody followed by HRP-avidin conjugate and TMB substrate, with absorbance proportional to TXNIP concentration in each well. The reported product range is 0.156-10 ng/ml with a sensitivity of 0.066 ng/ml, and total assay time is 4h 30m. Kit components are stored at +4°C or -20°C as instructed.

Reported recovery values average 91% for serum (range 80-102%), 90% for EDTA plasma (range 81-100%), and 84% for heparin plasma (range 80-89%) across the tested sample matrices.